LMN-HM521-100μg / 詢價
LMN-HM521-100μgx5 / 詢價
LMN-HM521-100μgx10 / 詢價
- 客服電話
- 技術(shù)支持
Mammalian cells
≥95%
0.1 mg/ml
< EU/ml
Negative
PBS (pH 7.4)
-20°C to -80°C, stable for at least 2 years from date of receipt.
3 months at 2-8°C after thaw.
Laminin 521, a heterotrimeric glycoprotein composed of one alpha 5, one beta 2 and one gamma 1 chain, is a defined matrix for feeder-free culture of human embryonic (hES) and induced pluripotent (hiPS) stem cells. When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human hES and hiPS cells without spontaneous differentiation.
? Supports stable self-renewal of hPSCs
? Improved recovery and survival following passage of singularized PSCs in the absence of ROCK inhibitors
? MSC and other stem cell culture
? Maintain the differentiation potential of cells in long-term culture
? Other related applications
(1) Yap L, Tay HG, Nguyen MTX, Tjin MS, Tryggvason K. Laminins in Cellular Differentiation. Trends Cell Biol. 2019 Dec;29(12):987-1000.
(2) Rodin S, Antonsson L, Hovatta O, Tryggvason K. Monolayer culturing and cloning of human pluripotent stem cells on laminin-521-based matrices under xeno-free and chemically defined conditions. Nat Protoc. 2014 Oct;9(10):2354-68.
(3) Lu HF, Chai C, Lim TC, Leong MF, Lim JK, Gao S, Lim KL, Wan AC. A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells. Biomaterials. 2014 Mar;35(9):2816-26.
(4) Rodin S, Antonsson L, Niaudet C, Simonson OE, Salmela E, Hansson EM, Domogatskaya A, Xiao Z, Damdimopoulou P, Sheikhi M, Inzunza J, Nilsson AS, Baker D, Kuiper R, Sun Y, Blennow E, Nordenskj?ld M, Grinnemo KH, Kere J, Betsholtz C, Hovatta O, Tryggvason K. Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nat Commun. 2014;5:3195.
(5) Durbeej M. Laminins. Cell Tissue Res. 2010 Jan;339(1):259-68.
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